들깨(Perilla fruescens var.japonica (Hassk.).H.hara)처리에 따른 BV-2 세포의 차이유전자(DEG) 발현 dataset

48386_F049000B14a

들깨(Perilla fruescens var.japonica (Hassk.).H.hara)처리에 따른 BV-2 세포의 차이유전자(DEG) 발현 dataset

"RNASeq을 이용한 천연물처리에 따른 세포 전사체 분석 및 차이유전자 발굴 Comparing expression profiles between comparable samples using Transcriptome Resequecing data.

RNASeq, NGS, 전사체, 뇌세포

들깨_잎_재래종_S24044_180471(115)

1. Sample treatment - BV-2 microglial cell [DMEM , 10% FBS, 1% P/S ]/10,000 cell per well - Stimulation : LPS 500 ng/mL - Treated Perilla fruescens var.japonica (Hassk.).H.hara extracts (50μg/mL) and overnight incubation 2. RNA extraction and sequencing 3. Data Pre-processing & Quality Check : Filtering → RLE Normalization - Estimate size factor using read count data - Use Relative Log Expression (RLE) normalization - Use DESeq2 R library 4. Statistical Test : Fold Change, nbinomWaldTest using DESeq2 - Criteria for Significant Result : |fc|≥2 & raw.p<0.05 5. Gene-Enrichment and Functional Annotation Analysis : using gProfiler

Significant genes (들깨44 vs Control) : 303 genes [up 80, down 223] - Criteria for Significant Result : |fc|≥2 & raw.p<0.05

전라북도 고창군

장환희

김현정, 조수연

국립식량과학원

2025

내외부공개

1